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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2011 Dec 5;188(2):800–810. doi: 10.4049/jimmunol.1100769

Figure 3.

Figure 3

T. gondii-specific Th1 T cells produce limited amounts of IL-2

WT animals (five mice per group) were either left untreated (A) or infected with T. gondii intraperitoneally (B) or orally (C), and seven days later, the ability of splenic CD4+ T cells to produce IFN-γ, TNF, and IL-2 was analyzed. Analyses of intracellular cytokine expression were performed on splenocytes restimulated with 0.5 μg/ml αCD3 for 5 hr in the presence of GolgiPlug. (D) Expression levels of IFN-γ and (E) IL-2 were analyzed by real-time PCR in splenocytes, CD4+, CD8+, and CD4+Foxp3+ T cells isolated from naïve or T. gondii infected mice (day 7 post infection). (F) IL-2 in cell culture supernatant was measured by ELISA on splenocytes isolated from naïve (d0) or T. gondii infected mice at the indicated time points after restimulation with 0.01 ug/ml αCD3 for 48 hr. (G) Expression levels of CD25 were analyzed by real-time PCR in naïve or T. gondii infected mice (day 7) on the same samples as shown in (D and E). The data shown are representative of five independent experiments. * P< 0.05; ** P< 0.01, *** P< 0.001.