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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2011 Dec 7;188(2):844–853. doi: 10.4049/jimmunol.1101736

Figure 6.

Figure 6

IL-17A directly enhances PMN and macrophage inflammatory functions. A) PMN ex vivo exposure to IL-17A exhibit an accelerated infiltration during zymosan-induced peritonitis. Bone marrow leukocytes from healthy mice were treated for 2 h with a recombinant IL-17A (2ng/ml) or serum from mice with DSS-induced colitis (DSS, 12d) in the presence or absence of the neutralization anti-IL-17A antibody. After treatment, the cells were labeled with CSFE (green) and co-transferred with control, non-treated bone marrow leukocytes (red) into healthy recipient mice followed by testing for PMN infiltration during zymosan-induced peritonitis. B) IL-17A directly enhances PMN chemotactic transmigration. Bone marrow PMN with and without exposure to IL-17A were assayed in vitro for chemotaxis towards fMLF for 90 min. C) IL-17A promotes IL-6 production in macrophage upon stimulation. Peritoneal macrophages obtained from healthy mice were treated with recombinant IL-17A (2ng/ml) with or without the presence of anti-IL-17A antibody for 48 h (37°C). IL-6 production in cells with or without zymosan stimulation (2 h) was assayed by ELISA. D) Western blot analyses of protein phosphorylation of macrophages treated with IL-17A.