Skip to main content
. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Immunol. 2011 Dec 16;188(2):531–535. doi: 10.4049/jimmunol.1102703

Figure 2. Long-term persistence of increased MC numbers and enhanced systemic anaphylaxis in post-dermatitis mice.

Figure 2

(A) Protocol scheme and measurement of MC numbers in Balb/c mice after 5 weeks of post-dermatitis convalescence and concomitant 9 weeks of OVA treatment, as shown (n=5). **, p=0.0039; ***, p=0.0006. (B) Toluidine- blue (TB) staining for MCs in indicated tissues, 5 weeks after cessation of either vehicle (acetone) or oxazolone. Bars, 150 μm. (C) Numbers of IL2 producing (IL-2+) MCs in the spleen of mice with dermatitis (Oxaz) or after recovery (5wk post-Oxaz). *, p=0.03. (D) Passive systemic anaphylaxis in mice with oxazolone-induced dermatitis or following their recovery (post-dermatitis). After challenge, body temperature was recorded using a subcutaneous probe (n=5). ***, p<0.0001. (E) Representative picture (left) of toluidine blue stained MCs in the spleens of mice after sequential oxazolone-dermatitis and OVA-airway hyper-reactivity induction, solvent (Ace) challenge only, or OVA-airway hyper-reactivity induction only. Quantitation of MC numbers in spleens (n=5). *, p=0.017; ***, p<0.0001. Bars, 150 μm.