Skip to main content
. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2011 Nov 9;52(2):410–419. doi: 10.1016/j.freeradbiomed.2011.10.495

Figure 3. Bcl-2 co-immunoprecipitates with GSH following chemical cross-linking in CGNs, an interaction antagonized by the BH3 mimetic, HA14-1.

Figure 3

CGNs were pre-incubated with either vehicle (0.4% DMSO) or 20 μM HA14-1 for 2 h, after which they were cross-linked (x-link) with DSS, which was quenched upon lysing (see materials and methods). Lysates were immunoprecipitated (IP) with a monoclonal antibody to GSH. Immune complexes were resolved by SDS-PAGE and immunoblotted (IB) for Bcl-2. Mono=monomeric, dimer=dimeric, WCL=whole cell lysate.