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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2011 Nov 9;52(2):410–419. doi: 10.1016/j.freeradbiomed.2011.10.495

Figure 6. Co-transfection with Bcl-2 and V5-OGC significantly increases mitochondrial glutathione content.

Figure 6

A. CHO cells were transfected with control (DsRed), Bcl-2, V5-OGC, or a combination of Bcl-2 and V5-OGC (5μg each). After 24 h, cells were fractionated and mitochondrial glutathione (GSH+GSSG) content was measured using DTNB, and normalized to protein concentration. Data are expressed as percent of control glutathione and analyzed using a one-way ANOVA with a post-hoc Tukey’s analysis *p<0.05 vs Bcl-2/OGC, n=3. Control levels of total mitochondrial GSH were at an average of 12.69 +/− 1.81 nmoles/mg of protein. B. Immunoblots of mitochondrial fractions indicating expression of Bcl-2, V5-OGC, or a combination of both in transfected CHO cells. C. Immunoblot for Cox-IV indicating purity of mitochondrial and cytosolic fractions.