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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Hepatology. 2011 Dec 29;55(2):437–446. doi: 10.1002/hep.24708

Figure 6. The effects of chronic ethanol feeding on lipin-1 in mouse livers.

Figure 6

(A) Relative levels of lipin-1 mRNA from mice fed a control diet with or without ethanol. (B) Acetylation of Histone H3-Lys9 was evaluated by Western blot using specific acetyl antibodies as indicated. (C) ChIP assays were performed. Immunoprecipitations (IP) were carried out using antibody directed against acetyl-Histone H3-lys9 from liver samples. Bound and input DNA was analyzed with primers for the Lpin1-SRE promoter by qRT-PCR. (D) Lipin-1 was immunoprecipitated from liver nuclear extracts of mice fed with control diet or ethanol and then immunoblotted with either an anti-acetylated lysine or anti-SUMO-1 antibody to determine the extent of lipin-1 acetylation or sumoylation or with a lipin-1 antibody to determine the total amount of lipin-1. IgG antibodies were used as a negative control. All data are expressed as means±SD; n=4–6 animals. Means without a common letter differ, p< 0.05