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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2011 Nov 9;52(2):452–461. doi: 10.1016/j.freeradbiomed.2011.10.489

Figure 2. Increase in invasive ability and MMP enzymatic activity of PC3 cells but not PrEC cells by incubation with a reducing extracellular redox state.

Figure 2

Cells were cultured with Cys/CySS supplemented media for 3 hr. Cell invasive ability through Matrigel matrix membrane and MMP activity were analyzed. (A) PrEC cell invasive ability. (B) PC3 cell invasive ability. (C) Total MMP activity in the media. (D) Zymography assay of MMP9. Purified pro-MMP9 proteins were directly incubated with EhCySS −180 mV medium and zymography enzyme activity was analyzed at different time points. *p-value ≤ 0.05.