Figure 5. Effects of decreased NADPH oxidase or MMP9 protein levels on PC3 cell invasion after culture in media with a reducing extracellular redox state.
PC3 cells were transfected with NOX1, MMP9, or non-specific siRNAs for 48 hr and continuously cultured for an additional 72 hr. siRNA transfected-PC3 cells were cultured with EhCySS −180 mV medium for 3 hr. Cells were collected for western blot (A and B) and in vitro invasion analysis (C). Mock= PC3 cells that were cultured with EhCySS −180 mV medium for 3 hr. *p-value ≤ 0.05.