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. 2011 Aug 26;438(Pt 3):523–533. doi: 10.1042/BJ20110607

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© 2011 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Fold-change of ENA1 expression deduced from microarray data for W303-1A wild-type cells (WT, empty bars) and MCY5278 (msn2 msn4, filled bars) exposed to pH 8.0 for the indicated times. Results are means±S.E.M. from four microarray experiments. (B) Strains with the indicated genotype (+, wild type allele; -, deletion mutant) were transformed with plasmid pKC201, which bears a LacZ fusion of the ENA1 promoter. Exponential cultures were resuspended in medium buffered at pH 5.5 (empty bars) or pH 8.0 (filled bars) for 1 h and β-galactosidase activity was measured. Results are means±S.E.M. from six to nine independent experiments.