Figure 2.

Panel A. Time- and dose-dependent inhibition of proliferation of cultured bovine pulmonary endothelial cells, BPAEC. Panel B. Fractionation of cytosolic cell extracts prepared from cultured BPAEC, with and without treatment for 48 h with 25 and 100 mM resveratrol, using resveratrol-immobilized affinity column. Lane marked MW, molecular weight marker; lanes 1-3 represent proteins eluted from affinity column using 1 mM ATP; lanes 4-6 represent proteins eluted from affinity column using 2 mM resveratrol; lane 7, unfractionated cytosolic extract from control cells. Lanes 1 and 4, eluted fractions from control cells; lanes 2 and 5, eluted fractions from cells treated with 25 mM resveratrol; lanes 3 and 6, eluted fractions from cells treated with 100 mM resveratrol. ATP and resveratrol eluted fractions were concentrated using a defined methanol:chloroform:water mixture [21, 47], separated by SDS-PAGE, and visualized by silver staining.