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. 2011 Jul 27;438(Pt 1):111–119. doi: 10.1042/BJ20101939

Figure 5. Endogenous FPP might regulate PPARγ activity during induction of adipocyte differentiation.

Figure 5

(A) Intracellular FPP levels in NIH 3T3 cells treated with or without lovastatin (Lova) or zaragozic acid (ZA). NIH 3T3 cells treated with vehicle control, 10 μM Lova or 1 μM ZA for 48 h were extracted with butanol/75 mM ammonium hydroxide/ethanol (1:1.25:2.75, by vol.) and the intracellular FPP level was determined as described in the Materials and methods section. All of the values are means±S.E.M. for three tests. (B) mRNA expression levels of aP2, LPL and adiponectin in 3T3-L1 cells treated with or without Lova or ZA. 3T3-L1 cells were induced to differentiate with vehicle control, Lova (5 or 10 μM) or ZA (0.5 or 1 μM) for 48 h. mRNA expression levels were determined as described in Figure 4. All the values are means±S.E.M. for four to six tests. *P<0.05, **P<0.01 compared with vehicle controls.