Table 1.
Unique properties of 1541 and related analogs compared to standard aggregators.
| Sample | Temp. (°C) | DLS Conc. (μM) | DLS Intensity (kcps) | Radius (nm) | Ksp (μM)† | AC50 (μM)‡ |
|---|---|---|---|---|---|---|
| Control | 25 | - | 19.7 ± 1.3 | 1.0 ± 0.3 | no particles | NA |
| Control | 37 | - | 20.8 ± 1.2 | 1.3 ± 0.3 | no particles | NA |
| 1541 | 25 | 10 | 270.2 ± 77.9 | 1112.8 ± 86.6 | 1.3 ± 0.3 | >50 |
| 1541 | 37 | 10 | 17.5 ± 1.4 | 0.5 ± 0.1 | 10 ± 5 | 3.0 ± 0.6 |
| 1541B | 25 | 10 | 170.1 ± 44.7 | 910.7 ± 66.7 | 1.6 ± 0.4 | 3.1 ± 0.5§ |
| 1541B | 37 | 10 | 15.8 ± 0.9 | 0.4 ± 0.1 | 20 ± 5 | 1.8 ± 0.1 |
| 1541D | 25 | 10 | 3317.0 ± 854.0* | 164.1 ± 7.7 | 7.5 ± 0.5 | NA |
| 1541D | 37 | 10 | 3071.9 ± 465.1* | 186.4 ± 21.6 | 7.5 ± 0.5 | NA |
| TIPT | 25 | 50 | 3903.5 ± 1423.0 | 71.1 ± 8.6 | - | NA |
| TIPT | 37 | 50 | 3933.2 ± 1369.0 | 76.7 ± 7.8 | - | NA |
*
1541D samples were run at 25% laser power.
†
Ksp values reported are from particle flow cytometry.
‡
Activity measurements were taken at 8 h.
§
Maximum percent activation for 1541B at 25°C is 8% compared to 60% at 37°C. NA = no activation.