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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Neurochem. 2011 Dec 8;120(3):419–429. doi: 10.1111/j.1471-4159.2011.07581.x

Figure 5.

Figure 5

Transfection with miR RNAi of PGC-1α exacerbated the impaired mitochondrial biogenesis in APPswe cells. A) Representative immunoblotting and quantification analysis showed that the expression of PGC-1α significantly reduced after transient transfection with miR RNAi of PGC-1α. B) Representative immunoblotting and quantification analysis revealed that the levels of NRF 1, NRF 2 and TFAM were significantly reduced after transient transfection (*p<0.05, Δp<0.01, Student's t test). C) Real time PCR revealed that mtDNA was significantly reduced after transient transfection with miR RNAi of PGC-1α for 48 h compared with EGFP control cells (Δp<0.01, Student's t test). D) Representative immunoblotting and quantification analysis revealed cytochrome B was significantly reduced (Δp<0.01, Student's t test) after cells was transfected with miR RNAi of PGC-1α for 48 h. E) Firefly luciferase assay showed that ATP level displayed a significant decrease after transient transfection with miR RNAi of PGC-1α for 48 h in APPswe cells (Δp<0.01, Student's t test). F) Cytochrome C oxidase activity was significantly decreased after transfection with miR RNAi of PGC-1α in APPswe cells (Δp<0.01, Student's t test).