Figure 6.
Effect of HO-1 inhibtion on microglia TNF-α (A) and IL-6 (B) mRNA. N9 microglia were pretreated for 3 h with vehicle (white bars) or HO-1 inhibitor (SnPP, 5 µM, small checkered bars), followed by 4 h (A) and 24 h (B) exposure to Mn (100 μM), LPS (100 ng/ml), or combined Mn+LPS. mRNA data are presented as fold change relative to control. a,b Letters indicate significant effects of Mn/LPS; * Indicates significant effect of HO-1 inhibitor within Mn/LPS (p ≤ 0.05).