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Effect of iNOS inhibition on HO-1 protein expression. N9 microglia were pretreated for 30 min with vehicle (white bars), L-NAME (2 mM, small checkered bars), or AG (25 μM, large checkered bars), followed by Mn (100 μM), LPS (100 ng/ml), or combined Mn+LPS for 24 h. Quantification and representative western blots are presented. Densitometric data were β-Actin-normalized and are presented as percent of control. a,b Letters indicate significant effects of Mn/LPS (p ≤ 0.05).
