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. Author manuscript; available in PMC: 2012 Dec 13.
Published in final edited form as: Circulation. 2011 Nov 14;124(24):2690–2701. doi: 10.1161/CIRCULATIONAHA.111.028498

Figure 1.

Figure 1

Figure 1

Successful disruption of SOCS3 and unregulated activation of gp130 downstream signaling in the heart. A, After 12 hours of serum starvation, isolated adult cardiomyocytes (8 weeks old) were stimulated with CT-1 (1nM) for the indicated time. The expression of SOCS3, phospho-STAT3 and endogenous STAT3 were estimated by western-blot (n=3 separate experiments). B, Known gp130 downstream signaling in the heart was examined at 8 and 15 weeks of age. The level of expression of each phospho-protein was normalized to the endogenous protein expression level. Expression level was calculated relative to the mean value of wild-type (WT) group (n=6 in each group). cKO; SOCS3 cKO * p<0.05 comparing SOCS3 cKO with WT hearts. Representative western-blot from three independent experiments is shown.