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. 2011 Nov 18;7:37. doi: 10.1186/1746-4811-7-37

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Copyright ©2011 Müller and Munné-Bosch; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of solvents on hormone extraction. Concentration of endogenous plant hormones (A) Z, DHZ, 2iP, IAA, ACC, and (B) ABA, JA, SA, GA₄, GA₉, GA₂₄detected in rosemary leaves using the following extraction solvents: M = methanol: glacial acetic acid, 99:1 (v/v); 80:20 M/I = methanol: isopropanol: glacial acetic acid 80:19:1 (v/v/v); 60:40 M/I = methanol: isopropanol: glacial acetic acid 60:39:1 (v/v/v); 40:60 M/I = methanol: isopropanol: glacial acetic acid 40:59:1 (v/v/v); 20:80 M/I = methanol: isopropanol: glacial acetic acid 20:79:1 (v/v/v); I = isopropanol: glacial acetic acid, 99:1 (v/v); 100:100 M/I = leaves were extracted first with methanol: glacial acetic acid, 99:1 (v/v) in two extraction steps and then with I = isopropanol: glacial acetic acid, 99:1 (v/v) in two extraction steps.