Box plots of median prostate cancer antigen 3 (PCA3) assay scores comparing men with and without (A) tumor volume < 0.5 mL; (B) pathologically insignificant prostate cancer (PCa); (C) extracapsular extension; (D) seminal vesicle invasion; and (E) pathologic Gleason score ≥ 7. Reproduced with permission from European Urology, Volume 59, Auprich M et al, “Critical assessment of preoperative urinary prostate cancer antigen 3 on the accuracy of prostate cancer screening,” pp. 96–105, Copyright 2011, with permission from Elsevier.19