Figure 3. Curcumin improves insulin stimulated PKB phosphorylation in fat tissue and hepatocytes.

(A) and (B) The three groups of mice fed with the diets as indicated for 28 weeks were fasted overnight and injected with PBS or insulin. After 30 min, the mice were sacrificed. Samples of epididymal fat pad (A) and liver (B) were prepared and immunoblotted with PKB or Ser473 phosphorylated PKB (p-PKB) antibody. (C) HepG2 cells were pre-treated with or without curcumin at indicated concentration overnight, followed by a 6 h treatment with or without glucose oxidase (GO). The cells were then further treated with or without insulin (100 nM) for 10 min, followed by PKB and p-PKB immunoblotting. The blots shown are representative of 3 separated experiments.