Figure 4. Curcumin does not stimulate the Wnt signaling pathway in mature adipocytes.

(A) Samples from epididymal fat pad of the three groups of mice were prepared for Western blotting. β-cat and GSK-3 represent Wnt pathway effectors, while cyclin D1 and c-Myc are two known Wnt target genes. (B) Rat primary adipocytes were prepared and treated with insulin (100 nM), or curcumin (20 µM), or insulin plus curcumin for 0, 5, 30, and 60 min. Samples were collected for Western blotting, with indicated antibody. (C) Rat primary adipocytes were prepared and treated with 10 or 20 µM curcumin for 4 h, followed by Western blotting with indicated antibody. All panels show the representative blot (n = 3).