Figure 4. Maximum likelihood tree based on concatenation of GAPDH, actin, EF-1α, and SSU rRNA gene sequences and rooted by Trimastix.

Unambiguously aligned amino acid sites of GAPDH (257), actin (268), and EF-1α (274), and nucleotide sites of SSU rRNA (1338) gene sequences were concatenated and analyzed in 28 parabasalian species with Trimastix as an outgroup. The tree was estimated in RAxML using the CAT model (CATMIX). The parameters and branch length were optimized for each gene partition individually. The supporting values (bootstrap in RAxML/Bayesian posterior probability) are indicated at the nodes. Values below 50% or 0.5 are indicated by hyphens. Vertical bars to the right of the tree represent the parabasalian classes. The scale bar corresponds to 0.10 substitutions per site.