Figure 4. Western blots of 17β-HSD1 (A) and 17β-HSD2 (B) with over expressing in MCF-7.

100,000 cells were seeded onto 6-well plates, the cells were transfected with plasmid with over expressing of 17β-HSD1 and 17β-HSD2, then western blot was carried out after 48 hours. GADPH was chosen as the internal control protein.