Figure 2. Silique development and seed-set of siz1-2, nahG siz1-2, wild-type, and the Prosiz1::SIZ1-GFP construct-transformed siz1-2 mutant plants (SSG).
(A) Siliques of siz1-2, nahG siz1-2, wild-type, and SSG 8–10 days after pollination. (B) Dissected silique from siz1-2 homozygous plants showing severely reduced seed-set and undeveloped ovules. Similar results were also found in line siz1-3 (data not shown). De, defective embryo. (C) Dissected silique from nahG siz1-2 plants showing severely reduced seed-set and undeveloped ovules, similar to siz1-2. De, defective embryo. (D) Dissected silique of a wild-type plant with a full seed-set. (E) Dissected silique of a SSG plant with full seed-set, similar to that of the wild-type plant. (F) Percentage of defective embryos in siz1-2, nahG siz1-2, Col-0, and SSG pistils. A mean value of three repeats, asterisks indicate no significant difference between percentage of defective embryos of siz1-2 and nahG siz1-2 (P<0.05).