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. 2012 Jan 9;7(1):e29970. doi: 10.1371/journal.pone.0029970

Figure 1. Separation of CF by ConA affinity chromatography and DEAE-Sepharose chromatography yielded a fraction with potent apoptotic activity in human monocytes.

Figure 1

A. Apoptosis induced by DEAE-Sepharose fractions 1 to 7 (F1 to F7) and unfractionated CF was measured by flow cytometry and presented as percentage of human monocytes that stained annexinV positive *** significance p<0.001, ** significance p<0.01, * significance p<0.05 as compared to unstimulated control. B. SDS-PAGE and silver staining of fractions of CF after ConA affinity (CF-Man) and DEAE-Sepharose chromatography (F1 to F7). Mw denotes the molecular mass standards.