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. 2012 Jan 9;7(1):e29970. doi: 10.1371/journal.pone.0029970

Figure 5. The M. tuberculosis RNA's activity is caspase-8 dependent.

Figure 5

A. Flow cytometry demonstrated M. tuberculosis H37Rv RNA (gpRNA) activated caspase-8. Human monocytes were stimulated with 1 µg/ml of purified M. tuberculosis H37Rv RNA gpRNA (open histogram with dotted line) or PPD (open histograms with solid line) for 48 h and caspase activity was determined by flow cytometry after staining with FLICA-YVAD-FMK (caspase-1), FLICA-DEVD-FMK (caspase-3), or FLICA-LETD-FMK (caspase-8). Tissue culture medium without RNA or PPD was used as the control (closed histogram). B. Stimulation of apoptosis based on annexinV staining was measured for monocytes stimulated with gpRNA or PPD (top histogram) and when the monocytes were pretreated for 1 h with 10 nM caspase-1 (YVAD-FMK), caspase-3 (DEVD-FMK), or caspase-8 (LETD-FMK) inhibitors (lower histograms).