Figure 3.

Cellulose-binding assay. Recombinant forms (all highly expressed in E. coli) of the designated CBM3s (20 µg) were mixed in predetermined amounts with 5 mg insoluble cellulose. The suspension was centrifuged and the pellet was washed (bound CBM3); the supernatant fluids (unbound CBM3) were collected, mixed with sample buffer and aliquots corresponding to 10% of the bound and unbound fractions were subjected to SDS–PAGE. Each experiment was repeated at least three times. The partition of the CBM3 bands between the bound and unbound states is shown. BSA was used as a negative control in all experiments.