Figure 1.
SDS–PAGE analysis of β-Gal purification. Lane M, molecular markers (labelled in kDa); lane 1, culture supernatant; lane 2, β-Gal after Phenyl Sepharose purification; lane 3, β-Gal after Q Sepharose purification; lane 4, β-Gal after Endo Hf endoglycosidase treatment; lane 5, β-Gal after aminophenyl β-d-thiogalactopyranoside affinity purification; lane 6, β-Gal after limited trypsinization; lane 7, β-Gal after Pros HS purification; lane 8, β-Gal after Superdex 200 gel filtration. Deglycosylated β-Gal precursor and mature β-Gal are indicated by arrows.