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. Author manuscript; available in PMC: 2012 Jan 9.
Published in final edited form as: Mol Cancer Res. 2010 Aug 24;8(10):1375–1387. doi: 10.1158/1541-7786.MCR-09-0537

FIGURE 2.

FIGURE 2

FIGURE 2

FIGURE 2

FIGURE 2

Smad3 and phospho-Smad3 localization in cyclin D-overexpressing MCF7 and T47D cells. Smad3 localized to the nucleus in MCF7 (A, B) and T47D (B, C) parental, vector control (V), and cyclin D-overexpressing (CD1) cell lines. Endogenous Smad3 and phospho-Smad3 were visualized by immunofluorescence microscopy with an anti-Smad3 polyclonal antibody and FITC-conjugated secondary antibody. Cell nuclei were counterstained with DAPI. Scale bar is 10 μm. Arrows denote nuclei. Arrowheads denote cytoplasmic Smad3.