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. Author manuscript; available in PMC: 2012 Jan 9.

Published in final edited form as: Mol Cancer Res. 2010 Aug 24;8(10):1375–1387. doi: 10.1158/1541-7786.MCR-09-0537

Relative transcriptional activity of various Smad3 constructs in cyclin D1 overexpressing MCF7 (A), T47D (B), and Hs578T (C) cell lines. Cells were co-transfected with the Smad-responsive CAGA-luc reporter construct and Renilla luciferase reporter, in addition to the indicated Smad3 expression constructs. Firefly and Renilla luciferase activities were determined. Data are shown as fold increase in normalized luciferase activity (firefly/Renilla) compared with empty vector-transfected MCF7, T47D, or Hs578T cells. Error bars indicate standard deviation from the mean of normalized luciferase activity for each study condition.

Relative transcriptional activity of various Smad3 constructs in cyclin D1 overexpressing MCF7 (A), T47D (B), and Hs578T (C) cell lines. Cells were co-transfected with the Smad-responsive CAGA-luc reporter construct and Renilla luciferase reporter, in addition to the indicated Smad3 expression constructs. Firefly and Renilla luciferase activities were determined. Data are shown as fold increase in normalized luciferase activity (firefly/Renilla) compared with empty vector-transfected MCF7, T47D, or Hs578T cells. Error bars indicate standard deviation from the mean of normalized luciferase activity for each study condition.