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. Author manuscript; available in PMC: 2012 Jan 9.
Published in final edited form as: Mol Cancer Res. 2010 Aug 24;8(10):1375–1387. doi: 10.1158/1541-7786.MCR-09-0537

FIGURE 5.

FIGURE 5

Dose-dependent increase in Smad3 transcriptional activity in MCF7 and T47D cells treated with increasing concentrations of CDK2 or CDK4 inhibitors. MCF7 (A) or T47D (B) study cell lines were transfected with the Smad-responsive CAGA-luc reporter construct, Renilla luciferase reporter, and wild type (WT) Smad3 expression vector, then treated with the indicated concentrations of CDK2 inhibitor (CDK2i) or CDK4 inhibitor (CDK4i). Firefly and Renilla luciferase activities were determined. Data are shown as fold increase in normalized luciferase activity (firefly/Renilla) compared with empty vector-transfected MCF7 or T47D cells. Error bars indicate standard deviation from the mean of normalized luciferase activity for each study condition.