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. Author manuscript; available in PMC: 2012 Jan 9.

Published in final edited form as: Mol Cancer Res. 2010 Aug 24;8(10):1375–1387. doi: 10.1158/1541-7786.MCR-09-0537

Inhibition of CDK4-mediated phosphorylation of Smad3 leads to G1 arrest in MCF7 parental and cyclin D-overexpressing cell lines. (A) MCF7 and MCF7-CD1 cells were transfected with empty vector (V), wild type Smad3 (S), or the T178 Smad3 mutant expression vectors. (B) T47D and T47D-CD1 cells were transfected with empty vector (V), wild type Smad3 (S), or the T8 Smad3 mutant expression vectors. The cells were then treated with vehicle or CDK4 inhibitor (I). Cells were harvested and the cell cycle profile was assessed by FACS analysis. Figures show one representative experiment from 3 separate data sets.

Inhibition of CDK4-mediated phosphorylation of Smad3 leads to G1 arrest in MCF7 parental and cyclin D-overexpressing cell lines. (A) MCF7 and MCF7-CD1 cells were transfected with empty vector (V), wild type Smad3 (S), or the T178 Smad3 mutant expression vectors. (B) T47D and T47D-CD1 cells were transfected with empty vector (V), wild type Smad3 (S), or the T8 Smad3 mutant expression vectors. The cells were then treated with vehicle or CDK4 inhibitor (I). Cells were harvested and the cell cycle profile was assessed by FACS analysis. Figures show one representative experiment from 3 separate data sets.