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Published in final edited form as: Virology. 2011 Dec 20;423(1):77–88. doi: 10.1016/j.virol.2011.11.019

Fig. 1 — Phylogenetic analysis of the M and PB1 genes of the H4N8 viruses isolated from gulls and stints in Japan. Full-length nucleotide sequences of the viral genes were phylogenetically analyzed in comparison with the sequences available in GenBank. (A) Analysis of the M gene. All the strains in this figure are H4N8 subtype viruses. The new H4N8 isolates are related to the Australian lineage, in which all other H4N8 viruses have originated from red-necked stints in Australia. (B) Analysis of the PB1 gene. The PB1 genes of the new H4N8 isolates are genetically distant from that of A/red-necked stint/Australia/1/04. The H4N8 viruses isolated in this study are highlighted by circles, and A/red-necked-stint/Australia/1/04 (H4N8) and its related strains are highlighted with squares. The numbers in the tree represent bootstrap values (1,000 replicates).

Fig. 1 — Phylogenetic analysis of the M and PB1 genes of the H4N8 viruses isolated from gulls and stints in Japan. Full-length nucleotide sequences of the viral genes were phylogenetically analyzed in comparison with the sequences available in GenBank. (A) Analysis of the M gene. All the strains in this figure are H4N8 subtype viruses. The new H4N8 isolates are related to the Australian lineage, in which all other H4N8 viruses have originated from red-necked stints in Australia. (B) Analysis of the PB1 gene. The PB1 genes of the new H4N8 isolates are genetically distant from that of A/red-necked stint/Australia/1/04. The H4N8 viruses isolated in this study are highlighted by circles, and A/red-necked-stint/Australia/1/04 (H4N8) and its related strains are highlighted with squares. The numbers in the tree represent bootstrap values (1,000 replicates).