Table 5.

T_m-values of duplexes between centrally modified ONs and complementary or centrally mismatched RNA targets.^[a]

		Tm (ΔTm) [°C]	Mismatch ΔTm [°C]
ON	Sequence	B= A	C	G	U
2	5′-CGCAA CTC AACGC	51.5	−15.5	−3.0	−13.5
4	5′-CGCAA TTT AACGC	40.5	−19.0	−3.5	−17.0
6	5′-CGCAA CWC AACGC	47.0 (−4.5)	+1.0	+0.0	+0.5
8	5′-CGCAA TWT AACGC	42.0 (+1.5)	+3.0	+0.5	+1.5
12	5′-CGCAA TXT AACGC	38.0 (−2.5)	+1.0	+0.0	+1.0
14	5′-CGCAA CYC AACGC	43.5 (−8.0)	−2.0	−5.0	−4.0
16	5′-CGCAA TYT AACGC	36.0 (−4.5)	+1.5	−1.0	−1.0
18	5′-CGCAA CZC AACGC	44.5 (−7.0)	−3.0	−8.0	−7.0
20	5′-CGCAA TZT AACGC	36.5 (−4.0)	−12.0	−7.5	−13.0

^[a] Conditions and definitions as described in footnote of Table 1. RNA targets: 3′-GCGUU GBG UUGCG (for ON2/ON6/ON14/ON18) and 3′-GCGUU ABA UUGCG (for ON4/ON8/ON12/ON16/ON20).