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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Melanoma Res. 2012 Feb;22(1):19–29. doi: 10.1097/CMR.0b013e32834d2506

Figure 2. High dose IL-2 upregulates IL-24 expression and secretion in IL-24 positive melanoma cells.

Figure 2

IL-24 expression in melanoma cells treated with IL-2 (1000 U/ml), for 24 hours was determined by immunoblotting with anti IL-24 monoclonal (7G11)(A).Treatment with LPS (100 ng/ml) was included as a positive control. The graph shows relative IL-24 expression levels determined by densitometry and normalized against β-actin expression using Scion Image Software. *P < 0.05 compared to cells without IL-2 stimulation. These experiments (A) were carried out five times. Shown are mean +/− SE of these results. Levels of secreted IL-24 were determined by ELISA in cells treated with IL-2 (1000 U/ml) (B). Pre-treatment with anti-hIL-2 antibody (1μg/ml) blocked this response in WM1341 (C) as shown by Western analysis with expression levels determined by densitometry and normalized againstβ-actin expression (C).