Table 1.
IL-24 and IL-2R chain PCR primers and efficiency
| hIL-24 (IL-24) accession #: NM_006850 |
1484(+) AAGCAGATCCTCAATAAACATTTC 1556(−) ACCAAGGGAAAGGGATGATG PCR amplicon length =77 bases Lowest limit of detection = 190 copies PCR efficiency = 98% |
| hIL-2Ra (IL2RA) accession #: NM_000417 |
1061(+) CTAAATGGTCGCCCAGGAG 1128(−) TGTGATGTGACTTCAGAGCTT PCR amplicon length = 90 bases Lowest limit of detection = 160 copies (RT-PCR) PCR efficiency = 99% |
| hIL-2Rb (IL2RB) accession #: NM_000878 |
2037(+) CTCCCTCGTTAATCACAGGAT 2124(−) AGGACTGATATTGGTGAATAGCT PCR amplicon length = 90 bases Lowest limit of detection = 160 copies (RT-PCR) PCR efficiency = 99% |
| hIL-2Rg (IL2RG) accession #: NM_000206 |
380(+) CCACCTCTACCAAACATTTGTT 450(−) GCATCTGTGTGGCCTGTC PCR amplicon length = 80 bases Lowest limit of detection = 180 copies (RT-PCR) PCR efficiency = 99% |