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a qPCR amplification in a dilution series of the plasmid pCR4-TOPO cloning vector containing the inserted gltA fragment of Rickettsia helvetica (standard type). b Standard calibration curve for R. helvetica plasmid pCR4-TOPO cloning vector showing linearity in the dilution series between 1.5 and 1.5 × 108 copies (Error 0.0142, Efficiency 1.961, R2 0.997, y=−3.34x+37.68)
