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. 2011 Dec 29;2011:846096. doi: 10.1155/2011/846096

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Copyright © 2011 Qi Ru Wang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of cytokines in EPC-CM. The culture media of EPC colonies were collected after 48-hour incubation in serum-free condition. (a) Concentrations of cytokines in supernatant were measured by ELISA. (b) Ten thousand cells were cultured in wells of 24-well plate with EPC-CM (closed circle) or HUVEC-CM (open circle) of the following concentrations: 0%, 5%, 10%, 20%, and 40%, respectively. The number of endothelial cell colonies was counted after 14-day culture. The mean ±SD of triplicates-presented at each point in comparison to HUVEC-CM group. *P < 0.05. VEGF: vascular endothelial growth factor, GM-CSF: granulocyte-macrophage colony-stimulating factor.