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Analysis of IgG1 (a) and IgG2a (b) antibody responses of BALB/c mice to outer membrane vesicles from Brucella. Outer membrane vesicles (OMVs) were purified from B. melitensis 16 M and B. melitensis VTRM1, and mice were immunized. Sera from each mouse were collected and were assayed individually by ELISA. Antibody levels are expressed as optical density (OD) at 492 nm.
