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. Author manuscript; available in PMC: 2012 Apr 28.
Published in final edited form as: Neuron. 2011 Apr 28;70(2):326–338. doi: 10.1016/j.neuron.2011.01.031

Figure 1.

Figure 1

Paired pulse paradigms suggest vesicle trafficking is fast, limiting the ability to identify discrete pools. (A) Paired pulses of 300 ms duration with interpulse intervals from 1000 to 10 ms, capacitance shown above and ICa below. Red lines indicate instantaneous capacitance values, revealing a lack of depletion regardless of interpulse interval (IPI) with short duration IPIs unmasking a facilitatory effect. (B) Summary of data from protocol shown in (A) demonstrating that release from the second stimulus was greater than that observed in the first (n = 4). Bar graph represents change in capacitance for second pulse with IPI given below, ctrl is the average capacitance change for the first stimulus. (C) Summary of release data where the IPI was constant at 30 ms and the stimulus duration varied from 10 to 300 ms. Cross hatched bars indicate second pulse response, while open bars indicate initial response, n is given above each pair. Asterisks indicate statistical significance at the p<0.05 level.