Table 1.
Antibodies | Host° | Dilution* | Source | Characterization |
---|---|---|---|---|
VGAT | Rabbit | 1:500 (IF) | Synaptic system/131003 | Takamori et al. (2000) |
1:1000 (WB) | ||||
1:500 (II) | ||||
VGAT | Mouse | 1:50 (IF) | Synaptic system/131011 | Bogen et al. (2006), Tafoya et al. (2006) |
1:500 (WB) | ||||
VGLUT1 | Guinea pig | 1:2000 (IF and WB) | Chemicon/AB5905 | Melone et al. (2005) |
VGLUT1 | Rabbit | 1:500 (II) | Synaptic system/135303 | Takamori et al. (2001) |
VGLUT2 | Guinea pig | 1:2000 (IF and WB) | Chemicon/AB5907 | Cubelos et al. (2005), Liu et al. (2005) |
VGLUT2 | Rabbit | 1:500 (II) | Synaptic system/135403 | Takamori et al. (2001) |
SYT1 | Mouse | 1:1500 (IF and WB) | Synaptic system/105011 | Brose et al. (1992), Von Kriegstein et al. (1999) |
SYT2 | Rabbit | 1:800 (IF) | Synaptic system/105123 | Johnson et al. (2010) |
1:1000 (WB) | ||||
SV2A | Rabbit | 1:1500 (IF) | Synaptic system/119002 | Janz and Sudhof (1999) |
1:80.000 (IF-TSA) | ||||
1:1000 (WB) | ||||
SV2B | Rabbit | 1:1500 (IF) | Synaptic system/119102 | Janz and Sudhof (1999) |
1:80.000 (IF-TSA) | ||||
1:1000(WB and EM) | ||||
Rab3a | Mouse | 1:1500 (IF) | Synaptic system/107111 | Matteoli et al. (1991) |
1:1000 (WB) | ||||
Rab3c | Rabbit | 1:1500 (IF) | Synaptic system/107203 | Cai et al. (2008) |
1:1000 (WB) |
°GP, guinea pig; M, mouse; R, rabbit; *IF, immunofluorescence; IF-TSA, immunofluorescence with tyramide signal amplification; WB, western blotting; II, immunoisolation; EM, electron microscopy. Western blotting studies for VGLUT1, SYT1, SYT2, SV2A, and SV2B were performed with 5 μg of protein, those for VGAT, VGLUT2, Rab3a, and Rab3c with 10 μg.