Table 1.

Primary antibodies.

Antibodies	Host°	Dilution*	Source	Characterization
VGAT	Rabbit	1:500 (IF)	Synaptic system/131003	Takamori et al. (2000)
		1:1000 (WB)
		1:500 (II)
VGAT	Mouse	1:50 (IF)	Synaptic system/131011	Bogen et al. (2006), Tafoya et al. (2006)
		1:500 (WB)
VGLUT1	Guinea pig	1:2000 (IF and WB)	Chemicon/AB5905	Melone et al. (2005)
VGLUT1	Rabbit	1:500 (II)	Synaptic system/135303	Takamori et al. (2001)
VGLUT2	Guinea pig	1:2000 (IF and WB)	Chemicon/AB5907	Cubelos et al. (2005), Liu et al. (2005)
VGLUT2	Rabbit	1:500 (II)	Synaptic system/135403	Takamori et al. (2001)
SYT1	Mouse	1:1500 (IF and WB)	Synaptic system/105011	Brose et al. (1992), Von Kriegstein et al. (1999)
SYT2	Rabbit	1:800 (IF)	Synaptic system/105123	Johnson et al. (2010)
		1:1000 (WB)
SV2A	Rabbit	1:1500 (IF)	Synaptic system/119002	Janz and Sudhof (1999)
		1:80.000 (IF-TSA)
		1:1000 (WB)
SV2B	Rabbit	1:1500 (IF)	Synaptic system/119102	Janz and Sudhof (1999)
		1:80.000 (IF-TSA)
		1:1000(WB and EM)
Rab3a	Mouse	1:1500 (IF)	Synaptic system/107111	Matteoli et al. (1991)
		1:1000 (WB)
Rab3c	Rabbit	1:1500 (IF)	Synaptic system/107203	Cai et al. (2008)
		1:1000 (WB)

°GP, guinea pig; M, mouse; R, rabbit; *IF, immunofluorescence; IF-TSA, immunofluorescence with tyramide signal amplification; WB, western blotting; II, immunoisolation; EM, electron microscopy. Western blotting studies for VGLUT1, SYT1, SYT2, SV2A, and SV2B were performed with 5 μg of protein, those for VGAT, VGLUT2, Rab3a, and Rab3c with 10 μg.