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Profile of nutrient consumption and by-product formation during high-density transfection of Hek293 cells. Cells were re-suspended in 20 mL of fresh HyQSFM4TransFx293 medium to a final density of 5 × 106 cells/mL and transfected using a PEI:DNA ratio of 2:1. (A) Transfection using 0.4, 0.6, and 0.8 μgDNA/106 cells at 37°C; (B) Repeated transfection using 0.4 μgDNA/106 cells at 0, 48, and 96 hours post-inoculation at 37°C and 34°C.
