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High-density transfection of Hek293 cells in batch mode (without changing the medium). Cells were re-suspended in 20 mL of fresh HyQSFM4TransFx293 medium to a final density of 5 × 106 cells/mL and transfected with 2 μg DNA and 4 μg PEI in 125 mL Erlenmeyer flask at 37°C.
