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. 2012 Jan 10;2:266. doi: 10.3389/fmicb.2011.00266

Figure 2.

Figure 2

ΔesxKL mutant is defective in intracellular growth. (A) THP-1 cells were infected with wild-type (open squares) or ΔesxKL mutant (open triangles) at a MOI of 1: l. Data points indicate the mean number of CFUs for three independently infected wells and error bars indicate SD from means. Intracellular growth of the ΔesxKL mutant was significantly reduced in three independent experiments (P < 0.005)* at 96 h as determined by unpaired t-test. (B) THP-1 cells were infected at MOI of 1:1 and a growth index as CFU at 96 h relative to CFU at 24 h was determined for wild-type (open bar), ΔesxKL (light gray bar), esxK complemented (dark gray bar), and esxL complemented (black bar) strains. Reduced intracellular growth of ΔesxKL (P < 0.005)* was reversed by restoring only esxL. Data shown is a representative of two independent experiments. (C) The ΔesxKL mutant showed this growth defect in similar experiments with a primary single-donor PBMC infection model, and restoring esxL allowed normal intracellular growth.