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Reduced in vitro growth of ΔesxKL mutant. Cultures were grown in M7H9 broth supplemented with 0.05% Tween and OADC and optical density measurements taken at 600 nm. Circles indicate optical density for wild-type, squares for ΔesxKL mutant, and triangles for esxL complemented mutant strain. The reduced growth rate of the mutant was restored to wild-type levels by complementing with esxL. A representative of three experiments is shown.
