Table 1.
Mycobacterium tuberculosis ORFs corresponding to mRNAs detected either in four or five sputum specimens and potentially expressed in response to growth in the lung.
| Gene name | Synonym | Gene description | No. of sputum specimens |
|---|---|---|---|
| ribC | Rv1412 | Probable riboflavin synthase | 5 |
| Rv0520 | Rv0520 | Probable methyltransferase | 5 |
| Rv0988 | Rv0988 | Possible conserved exported protein | 5 |
| Rv2626c | Rv2626c | Conserved hypothetical protein | 5 |
| cethA | Rv3854c | Monooxygenase | 5 |
| hsp | Rv0251c | Heat shock protein | 5 |
| Rv0779c | Rv0779c | Possible transmembrane protein | 5 |
| Rv0836c | Rv0836c | Hypothetical protein | 4 |
| lpqQ | Rv0835 | Predicted surface lipoprotein | 4 |
| Rv3362c | Rv3362c | Probable ATP/GTP binding protein | 4 |
| Rv2627c | Rv2627c | Conserved hypothetical protein | 4 |
| pstA2 | Rv0936 | Phosphate-transport ABC transporter | 4 |
| Rv3542c | Rv3542c | Hypothetical protein | 4 |
| ansP | Rv2127 | Probable asparagine permease | 4 |
| sigM | Rv3911 | Sigma factor SigM | 4 |
| glgX | Rv1564c | Probable maltooligotrehalose synthase | 4 |
| Rv3054c | Rv3054c | Conserved hypothetical protein | 4 |
| Rv0195 | Rv0195 | Two component transcriptional regulator | 4 |
| pcnA | Rv3907c | Probable polyA-polymerase | 4 |
| cysW | Rv2398c | Probable sulfate-transporter ABC transporter | 4 |
| Rv1767 | Rv1767 | Conserved hypothetical protein | 4 |
| Rv1045 | Rv1045 | Hypothetical protein/MΦ survival | 4 |
| dppC | Rv3664c | Probable dipeptide ABC transporter | 4 |
| Rv3737 | Rv3737 | Conserved transmembrane protein | 4 |
| Rv3779 | Rv3779 | Conserved transmembrane protein | 4 |
| Rv2567 | Rv2567 | Conserved transmembrane protein | 4 |
| Rv2293c | Rv2293c | Conserved hypothetical protein | 4 |
| Rv2823c | Rv2823c | Conserved hypothetical secreted protein | 4 |
| aceA | Rv0467 | Isocitrate lyase | 4 |
The Table lists arrays features that showed hybridization above background with SCOTS cDNA from sputum bacteria but not first-strand cDNA from mid-log broth cultures. Hybridization signal levels at least threefold over background were considered detection of the corresponding mRNAs. cDNA probes were prepared from shaking mid-log phase M7H9 OADC broth-grown cultures of M. tuberculosis H37Rv and sputum bacteria as described in Section “Materials and Methods.” ORFS are annotated as per Tuberculist (Genolist, Pasteur Institute).