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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Curr Protoc Protein Sci. 2011 Aug;CHAPTER:Unit17.13. doi: 10.1002/0471140864.ps1713s65

Figure 1.

Figure 1

(A) Setup of a homemade plunge-freezer. This basic freezing device that can easily be made by a local machine shop, has a height-adjustable metal plunger to which the forceps holding the grid can be attached and removed with a simple clip mechanism. Immediately after blotting excess fluid from the grid, the plunger is released by pushing the plunge controller. This injects the grid into a cup of ethane slush (magnified in B) that is kept cool by the liquid nitrogen in the outer foil-lined styrofoam box.