Table 3.
Iteration table with typical search parameters for aligning and averaging different types of samples using PEET. Search parameters are very dataset dependent and must be optimized for each specimen.
| Iteration Number | Angular search Range (degrees) | Search Radius (pixels) | ||||||
|---|---|---|---|---|---|---|---|---|
| SAMPLE | Phi | Theta | Psi | |||||
| Max | Incr. | Max | Incr. | Max | Incr. | |||
| Kinesin-decorated microtubules | 1 | 18.0 | 2.0 | 0.0 | 1.0 | 0.0 | 1.0 | 5 |
| 2 | 9.0 | 3.0 | 5.0 | 2.5 | 5.0 | 2.5 | 4 | |
| 3 | 4.5 | 1.5 | 3.2 | 1.6 | 3.2 | 1.6 | 2 | |
| 4 | 2.25 | 0.75 | 2.0 | 1.0 | 2.0 | 1.0 | 2 | |
| 5 | 1.125 | 0.375 | 1.2 | 0.6 | 1.2 | 0.6 | 2 | |
| Virus Particles | 1 | 36.0 | 9.0 | 36.0 | 9.0 | 36.0 | 9.0 | 3 |
| 2 | 12.0 | 3.0 | 12.0 | 3.0 | 12.0 | 3.0 | 2 | |
| 3 | 6.0 | 2.0 | 6.0 | 2.0 | 6.0 | 2.0 | 2 | |
| 4 | 3.0 | 1.0 | 3.0 | 1.0 | 3.0 | 1.0 | 2 | |