Figure 6. Binding assays of selected peptides from the randomized libraries with the hAT1R-expressing cells.

Selected streptavidin-fused peptides were generated by the PURE system. An aliquot of the reaction mixture was incubated with the hAT1R/CHO-K1 cells (hAT1R +) or the Mock/CHO-K1 cells (hAT1R −). The cells were washed, and the bound molecules were detected with anti-T7·tag antibody. The sequences of the peptides are shown in Figures 4A and 5A.