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. 2012 Jan 10;7(1):e30032. doi: 10.1371/journal.pone.0030032

Figure 1. RT-PCR reveals expression of adenosine receptors in brain, taste epithelium but limited expression in non-taste lingual epithelium.

Figure 1

Top: In the brain, all 4 adenosine receptors (A1R, A2AR, A2BR and A3R) are readily detected along with Gα14. Gαgust is not detectable. Center: In posterior taste fields (CV/FO) A2BR transcript is detected; a faint band is found for A1R in some preparations. Both Gαgust and Gα14 are also evident. Bottom: In non-gustatory epithelium (NTT = Non-Taste Tissue) A2BR but not Gαgust or Gα14 is detected. A faint band for A1R is also present in some preparations as in CV/FO epithelium. PCR products were visualized using UV illumination following ethidium bromide staining. Expression of mRNA for GAPDH was used as a positive control. The reverse transcriptase step was omitted as a negative control [(-)CONT] to confirm removal of all genomic DNA.